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pentr d topo vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pentr d topo vector
    Pentr D Topo Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+d+topo+vector/Topoisomerase+I/pmc12018254-180-16-25
    Average 96 stars, based on 448 article reviews
    pentr d topo vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
    Article Snippet: .. Cloned sequences were assembled with fragments encoding axr-mCherry and KUP5 or LRRAC1 together with the linearized pENTR/D-TOPO vector to generate the entry clone using the NEBuilder HiFi DNA Assembly Kit (NEB, catalogue no. E2621L). ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
    Article Snippet: .. Cloned sequences were assembled with fragments encoding axr-mCherry and KUP5 or LRRAC1 together with the linearized pENTR/D-TOPO vector to generate the entry clone using the NEBuilder HiFi DNA Assembly Kit (NEB, catalogue no. E2621L). ..

    Polymerase Chain Reaction:

    Article Title: Radip light-induced phosphorylation changes in microtubule related proteins in arabidopsis
    Article Snippet: .. The PCR products and the pENTR-D-TOPO vector were digested using the restriction enzymes NotI-HF y AscI (New England BioLabs) following the instructions of the supplier and the digested vector was dephosphorylated by TSAP enzyme to avoid its recircularization (Promega, Madison, WI, USA). .. After electrophoresis, all the fragments were purified using QIAEXII Gel Extraction Kit (QIAGEN, Germany).

    Article Title: Arabidopsis hypocotyl growth in darkness requires the phosphorylation of a microtubule-associated protein.
    Article Snippet: Received 26 July 2023; revised 23 February 2024; accepted 28 February 2024.. *For correspondence (e-mail mazzellaagus@gmail.com).. #These authors equally contributed to this work.

    Plasmid Preparation:

    Article Title: Radip light-induced phosphorylation changes in microtubule related proteins in arabidopsis
    Article Snippet: .. The PCR products and the pENTR-D-TOPO vector were digested using the restriction enzymes NotI-HF y AscI (New England BioLabs) following the instructions of the supplier and the digested vector was dephosphorylated by TSAP enzyme to avoid its recircularization (Promega, Madison, WI, USA). .. After electrophoresis, all the fragments were purified using QIAEXII Gel Extraction Kit (QIAGEN, Germany).

    Article Title: Arabidopsis hypocotyl growth in darkness requires the phosphorylation of a microtubule-associated protein.
    Article Snippet: Received 26 July 2023; revised 23 February 2024; accepted 28 February 2024.. *For correspondence (e-mail mazzellaagus@gmail.com).. #These authors equally contributed to this work.

    Article Title: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix
    Article Snippet: .. The constructs were subcloned into the pENTR-D-TOPO vector and afterwards brought into the expression vectors with EcoRI and XhoI (New England Biolabs), so that they were N -terminally tagged either with 6x Myc (pCS2+MT) or with the FLAG-tag (pCS2 + Flag) [ ]. ..

    Expressing:

    Article Title: The basic leucine zipper transcription factor OsbZIP83 and the glutaredoxins OsGRX6 and OsGRX9 facilitate rice iron utilization under the control of OsHRZ ubiquitin ligases.
    Article Snippet: Under low iron availability, plants induce the expression of various genes for iron uptake and translocation.. The rice (Oryza sativa) ubiquitin ligases OsHRZ1 and OsHRZ2 cause overall repression of these iron-related genes at the transcript level, but their protein-level regulation is unclear.. We conducted a proteome analysis to identify key regulators whose abundance was regulated by OsHRZs at the protein level.

    Article Title: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix
    Article Snippet: .. The constructs were subcloned into the pENTR-D-TOPO vector and afterwards brought into the expression vectors with EcoRI and XhoI (New England Biolabs), so that they were N -terminally tagged either with 6x Myc (pCS2+MT) or with the FLAG-tag (pCS2 + Flag) [ ]. ..

    Construct:

    Article Title: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix
    Article Snippet: .. The constructs were subcloned into the pENTR-D-TOPO vector and afterwards brought into the expression vectors with EcoRI and XhoI (New England Biolabs), so that they were N -terminally tagged either with 6x Myc (pCS2+MT) or with the FLAG-tag (pCS2 + Flag) [ ]. ..

    FLAG-tag:

    Article Title: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix
    Article Snippet: .. The constructs were subcloned into the pENTR-D-TOPO vector and afterwards brought into the expression vectors with EcoRI and XhoI (New England Biolabs), so that they were N -terminally tagged either with 6x Myc (pCS2+MT) or with the FLAG-tag (pCS2 + Flag) [ ]. ..



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